| Combination Therapy of Spinal Cord Injury in Rat with Minocycline and Bone Marrow Stromal Cells |
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Juno Park, Sang Dae Kim, Jang Bo Lee, Dong Jun Lim, Jung Yul Park, Young Gu Chung |
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Department of Neurosurgery, Korea University Medical Center, Ansan Hospital, Ansan, Korea |
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| Abstract |
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Bone marrow stromal cells (BMSCs) can be used as an alternative source of cells for neural transplantation. Improvement of neurological function was confirmed in animal model of spinal cord injury (SCI) after transplantation. Minocycline, a tetracycline derivative, mediates neuroprotection in experimental models of SCI. Author verified the hypothesis that combination therapy with BMSCs transplantation and minocycline will improve functional and pathological therapeutic result better than monotherapy after SCI. Rat spinal cords were compressed using aneurysmal clip, and neurological function was assessed at day 1, 7, 14, 21, and 28, using Basso-Beattie-Bresnahan locomotor rating scale. At day 28, rats were sacrificed, and their spinal cords were examined pathologically. At 7, 14, 21 and 28 day after spinal cord injury, the BMSCs transplantation group, minocycline treated group, and combination therapy group significantly (p<0.05) improved neurological outcome, compared with control group. But there were no significant differences among the treatment groups. Transplanted BMSCs differentiated into astrocytes and neurons. In all the treatment group, there was a small damage in the spinal cord in comparison with the control group. And there were significant differences between monotherapy group and combination therapy group on pathologic examination. These results suggest that combination therapy with BMSCs transplantation and minocycline injection may provide an additive therapeutic benefit after SCI
in animal model. |
| Key Words:
Bone marrow stromal cell, Combination therapy, Minocycline, Spinal cord injury, Transplantation. |
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